A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale

Bohland, Jason W, Wu, Caizhi, Barbas, Helen, Bokil, Hemant, Bota, Mihail, Breiter, Hans C, Cline, Hollis T, Doyle, John C, Freed, Peter J, Greenspan, Ralph J, Haber, Suzanne N, Hawrylycz, Michael, Herrera, Daniel G, Hilgetag, Claus C, Huang, Z Josh, Jones, Allan, Jones, Edward G, Karten, Harvey J, Kleinfeld, David, Kotter, Rolf, Lester, Henry A, Lin, John M, Mensh, Brett D, Mikula, Shawn, Panksepp, Jaak, Price, Joseph L, Safdieh, Joseph, Saper, Clifford B, Schiff, Nicholas D, Schmahmann, Jeremy D, Stillman, Bruce W, Svoboda, Karel, Swanson, Larry W, Toga, Arthur W, Essen, David C Van, Watson, James D, Mitra, Partha P (January 2009) A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale. PLoS Computational Biology. (Submitted)

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In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is however critical both for basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale of resolution suitable for comprehensive, brain-wide coverage, using injections of tracers or viral vectors. We detail the scientific and medical rationale and briefly review existing knowledge and experimental techniques. We define a set of desiderata, including brain-wide coverage; validated and extensible experimental techniques suitable for standardization and automation; centralized, open access data repository; compatibility with existing resources, and tractability with current informatics technology. We discuss a hypothetical but tractable plan for mouse, additional efforts for the macaque, and technique development for human. We estimate that the mouse connectivity project could be completed within five years with a comparatively modest budget.

Item Type: Paper
Subjects: organism description > animal > mammal > rodent > mouse
neurobiology > neuroscience
Investigative techniques and equipment > Whole Brain Circuit Mapping
CSHL Authors:
Communities: CSHL labs > Huang lab
CSHL labs > Mills lab
CSHL labs > Mitra lab
CSHL labs > Stillman lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 28 January 2009
Date Deposited: 13 Oct 2023 15:16
Last Modified: 22 Dec 2023 16:58
PMCID: PMC2655718
Related URLs:
URI: https://repository.cshl.edu/id/eprint/41241

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