Bohland, Jason W, Wu, Caizhi, Barbas, Helen, Bokil, Hemant, Bota, Mihail, Breiter, Hans C, Cline, Hollis T, Doyle, John C, Freed, Peter J, Greenspan, Ralph J, Haber, Suzanne N, Hawrylycz, Michael, Herrera, Daniel G, Hilgetag, Claus C, Huang, Z Josh, Jones, Allan, Jones, Edward G, Karten, Harvey J, Kleinfeld, David, Kotter, Rolf, Lester, Henry A, Lin, John M, Mensh, Brett D, Mikula, Shawn, Panksepp, Jaak, Price, Joseph L, Safdieh, Joseph, Saper, Clifford B, Schiff, Nicholas D, Schmahmann, Jeremy D, Stillman, Bruce W, Svoboda, Karel, Swanson, Larry W, Toga, Arthur W, Essen, David C Van, Watson, James D, Mitra, Partha P (January 2009) A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale. PLoS Computational Biology. (Submitted)
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Abstract
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is however critical both for basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale of resolution suitable for comprehensive, brain-wide coverage, using injections of tracers or viral vectors. We detail the scientific and medical rationale and briefly review existing knowledge and experimental techniques. We define a set of desiderata, including brain-wide coverage; validated and extensible experimental techniques suitable for standardization and automation; centralized, open access data repository; compatibility with existing resources, and tractability with current informatics technology. We discuss a hypothetical but tractable plan for mouse, additional efforts for the macaque, and technique development for human. We estimate that the mouse connectivity project could be completed within five years with a comparatively modest budget.
| Item Type: | Paper |
|---|---|
| Subjects: | organism description > animal > mammal > rodent > mouse neurobiology > neuroscience Investigative techniques and equipment > Whole Brain Circuit Mapping |
| CSHL Authors: | |
| Communities: | CSHL labs > Huang lab CSHL labs > Mills lab CSHL labs > Mitra lab CSHL labs > Stillman lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 28 January 2009 |
| Date Deposited: | 13 Oct 2023 15:16 |
| Last Modified: | 22 Dec 2023 16:58 |
| PMCID: | PMC2655718 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41241 |
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