Practical Considerations for Single-Cell Genomics

Regan, Claire, Preall, Jonathan (August 2022) Practical Considerations for Single-Cell Genomics. Curr Protoc, 2 (8). e498. ISSN 2691-1299

URL: https://www.ncbi.nlm.nih.gov/pubmed/35926125
DOI: 10.1002/cpz1.498

Abstract

The single-cell revolution in the field of genomics is in full bloom, with clever new molecular biology tricks appearing regularly that allow researchers to explore new modalities or scale up their projects to millions of cells and beyond. Techniques abound to measure RNA expression, DNA alterations, protein abundance, chromatin accessibility, and more, all with single-cell resolution and often in combination. Despite such a rapidly changing technology landscape, there are several fundamental principles that are applicable to the majority of experimental workflows to help users avoid pitfalls and exploit the advantages of the chosen platform. In this overview article, we describe a variety of popular single-cell genomics technologies and address some common questions pertaining to study design, sample preparation, quality control, and sequencing strategy. As the majority of relevant publications currently revolve around single-cell RNA-seq, we will prioritize this genomics modality in our discussion. © 2022 Wiley Periodicals LLC.

Item Type: Paper
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
Investigative techniques and equipment
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
CSHL Authors:
Communities: CSHL labs > Preall lab
CSHL Cancer Center Program
CSHL Cancer Center Program > Gene Regulation and Inheritance Program
CSHL Cancer Center Shared Resources > Single-Cell Biology Service
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: August 2022
Date Deposited: 23 Aug 2022 02:28
Last Modified: 09 Feb 2024 20:18
PMCID: PMC9479272
URI: https://repository.cshl.edu/id/eprint/40699

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