Cortical glutamatergic projection neuron types contribute to distinct functional subnetworks

Mohan, Hemanth, An, Xu, Kondo, Hideki, Zhao, Shengli, Matho, Katherine, Musall, Simon, Mitra, Partha, Huang, Z Josh (March 2022) Cortical glutamatergic projection neuron types contribute to distinct functional subnetworks. Research Square. (Unpublished)

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Abstract

The cellular basis of cerebral cortex functional architecture remains not well understood. A major challenge is to monitor and decipher neural network dynamics across broad cortical areas yet with projection neuron (PN)-type resolution in real time during behavior. Combining genetic targeting and wide-field imaging, we monitored activity dynamics of subcortical-projecting (PTFezf2) and intratelencephalic-projecting (ITPlxnD1) types across dorsal cortex of mice during different brain states and behaviors. ITPlxnD1 and PTFezf2 neurons showed distinct activation patterns during wakeful resting, spontaneous movements, and upon sensory stimulation. Distinct ITPlxnD1 and PTFezf2 subnetworks were dynamically tuned to different sensorimotor components of a naturalistic feeding behavior, and optogenetic inhibition of subnetwork nodes disrupted specific components of this behavior. Lastly, ITPlxnD1 and PTFezf2 projection patterns are consistent with their subnetwork activation patterns. Our results show that, in addition to the concept of columnar organization, dynamic areal and PN type-specific subnetworks are a key feature of cortical functional architecture linking microcircuit components with global brain networks.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex
bioinformatics > genomics and proteomics > small molecules > Glutamate
organs, tissues, organelles, cell types and functions > tissues types and functions > neural networks
CSHL Authors:
Communities: CSHL labs > Huang lab
CSHL labs > Mitra lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 15 March 2022
Date Deposited: 23 Mar 2022 19:43
Last Modified: 27 Nov 2023 15:40
PMCID: PMC10571488
URI: https://repository.cshl.edu/id/eprint/40557

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