Stec, Natalia, Doerfel, Katja, Hills-Muckey, Kelly, Ettorre, Victoria M, Ercan, Sevinc, Keil, Wolfgang, Hammell, CM (September 2020) An Epigenetic Priming Mechanism Mediated by Nutrient Sensing Regulates Transcriptional Output. BioRxiv. (Unpublished)
Abstract
While precise tuning of gene expression levels is critical for most developmental pathways, the mechanisms by which the transcriptional output of dosage-sensitive molecules is established or modulated by the environment remain poorly understood. Here, we provide a mechanistic framework for how the conserved transcription factor BLMP-1/Blimp1 operates as a pioneer factor to decompact chromatin near its target loci hours before transcriptional activation and by doing so, regulates both the duration and amplitude of subsequent target gene transcription. This priming mechanism is genetically separable from the mechanisms that establish the timing of transcriptional induction and functions to canalize aspects of cell-fate specification, animal size regulation, and molting. A key feature of the BLMP-1-dependent transcriptional priming mechanism is that chromatin decompaction is initially established during embryogenesis and maintained throughout larval development by nutrient sensing. This anticipatory mechanism integrates transcriptional output with environmental conditions and is essential for resuming normal temporal patterning after animals exit nutrient-mediated developmental arrests.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation |
| CSHL Authors: | |
| Communities: | CSHL labs > Hammell C. lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 3 September 2020 |
| Date Deposited: | 13 Jul 2021 20:16 |
| Last Modified: | 29 Apr 2024 15:43 |
| URI: | https://repository.cshl.edu/id/eprint/40285 |
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