Fein, MR, Wysocki, RW, Egeblad, M (January 2014) Cancer in the spotlight: Using intravital imaging in cancer research. In: Advances in Intravital Microscopy: From Basic to Clinical Research. Springer Netherlands, pp. 105-123. ISBN 9789401793605
Abstract
Intravital imaging can provide unique insights into cancer biology and has been particularly useful for research into therapy responses, metastasis, and immune responses. Here, we discuss the protocols that we routinely use for intravital imaging of mammary tumors to follow drug responses and for imaging of lungs to study metastatic seeding. These methods include: (1) the skin-flap technique used to image mammary tumors in a single, long experiment; (2) the use of an implantable imaging window for long-term serial imaging of mammary glands; and (3) the use of a thoracic window for intravital imaging of lungs to study the processes involved in the metastatic spread to lungs. We discuss common technical issues encountered when using each of these methods and provide our solutions for overcoming them. Finally, we discuss some of the exciting, new techniques on the horizon for intravital imaging in cancer biology.
| Item Type: | Book Section |
|---|---|
| Subjects: | Investigative techniques and equipment > imaging > intravital imaging diseases & disorders > cancer > cancer types > lung cancer diseases & disorders > cancer > drugs and therapies > tumor microenvironment |
| CSHL Authors: | |
| Communities: | CSHL labs > Beyaz lab CSHL labs > Egeblad lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 1 January 2014 |
| Date Deposited: | 02 Jun 2021 18:52 |
| Last Modified: | 02 Jun 2021 18:52 |
| URI: | https://repository.cshl.edu/id/eprint/40192 |
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