Li, Siran, Stone, Howard A, Murphy, Coleen T (January 2015) A microfluidic device and automatic counting system for the study of C. elegans reproductive aging. Lab on a Chip: miniaturisation for chemistry, physics, biology, materials science and bioengineering, 15 (2). pp. 524-531. ISSN 1473-0197
Abstract
The nematode Caenorhabditis elegans (C. elegans) is an excellent model to study reproductive aging because of its short life span, its cessation of reproduction in mid-adulthood, and the strong conservation of pathways that regulate longevity. During its lifetime, a wild-type C. elegans hermaphrodite usually lays about 200-300 self-fertilized hatchable eggs, which mainly occurs in the first three to five days of adulthood. Here, we report the development of a microfluidic assay and a real-time, automatic progeny counting system that records progeny counting information from many individual C. elegans hermaphrodites. This system offers many advantages compared to conventional plate assays. The flow of non-proliferating bacteria not only feeds the worms but also flushes the just-hatched young progeny through a filter that separates mothers from their offspring. The progeny that are flushed out of the chamber are detected and recorded using a novel algorithm. In our current design, one device contains as many as 16 individual chambers. Here we show examples of real-time progeny production information from wild-type (N2) and daf-2 (insulin receptor) mutants. We believe that this system has the potential to become a powerful, high time-resolution tool to study the detailed reproduction of C. elegans.
| Item Type: | Paper |
|---|---|
| Subjects: | organism description > animal > C elegans Investigative techniques and equipment > microscopy |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 21 January 2015 |
| Date Deposited: | 10 May 2021 13:24 |
| Last Modified: | 10 May 2021 13:24 |
| PMCID: | PMC4277648 |
| URI: | https://repository.cshl.edu/id/eprint/40070 |
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