Integrating barcoded neuroanatomy with spatial transcriptional profiling reveals cadherin correlates of projections shared across the cortex

Sun, Yu-Chi, Chen, Xiaoyin, Fischer, Stephan, Lu, Shaina, Gillis, Jesse, Zador, Anthony (August 2020) Integrating barcoded neuroanatomy with spatial transcriptional profiling reveals cadherin correlates of projections shared across the cortex. BioRxiv. (Unpublished)

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DOI: 10.1101/2020.08.25.266460

Abstract

Functional circuits consist of neurons with diverse axonal projections and gene expression. Understanding the molecular signature of projections requires high-throughput interrogation of both gene expression and projections to multiple targets in the same cells at cellular resolution, which is difficult to achieve using current technology. Here, we introduce BARseq2, a technique that simultaneously maps projections and detects multiplexed gene expression by in situ sequencing. We determined the expression of cadherins and cell-type markers in 29,933 cells, and the projections of 3,164 cells in both the mouse motor cortex and auditory cortex. Associating gene expression and projections in 1,349 neurons revealed shared cadherin signatures of homologous projections across the two cortical areas. These cadherins were enriched across multiple branches of the transcriptomic taxonomy. By correlating multi-gene expression and projections to many targets in single neurons with high throughput, BARseq2 provides a path to uncovering the molecular logic underlying neuronal circuits.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > auditory cortex
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organs, tissues, organelles, cell types and functions > tissues types and functions > motor cortex
organism description > animal > mammal > rodent > mouse
neurobiology > neuroanatomy
neurobiology
CSHL Authors:
Communities: CSHL labs > Gillis Lab
CSHL labs > Zador lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 26 August 2020
Date Deposited: 06 May 2021 19:38
Last Modified: 06 May 2021 19:38
URI: https://repository.cshl.edu/id/eprint/40031

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