A standardized and reproducible method to measure decision-making in mice.

The International Brain Laboratory, , Aguillon-Rodriguez, Valeria, Angelaki, Dora, Bayer, Hannah, Bonacchi, Niccolò, Carandini, Matteo, Cazettes, Fanny, Chapuis, Gaelle, Churchland, Anne, Dan, Yang, Dewitt, Eric, Faulkner, Mayo, Forrest, Hamish, Haetzel, Laura, Hausser, Michael, Hofer, Sonja, Hu, Fei, Khanal, Anup, Krasniak, Christopher, Laranjeira, Inês, Mainen, Zachary, Meijer, Guido, Miska, Nathaniel, Mrsic-Flogel, Thomas, Murakami, Masayoshi, Noel, Jean-Paul, Pan-Vazquez, Alejandro, Sanders, Josh, Socha, Karolina, Terry, Rebecca, Urai, Anne, Vergara, Hernando, Wells, Miles, Wilson, Christian, Witten, Ilana, Wool, Lauren, Zador, Anthony (January 2020) A standardized and reproducible method to measure decision-making in mice. BioRxiv. (Unpublished)

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URL: https://www.biorxiv.org/content/10.1101/2020.01.17...
DOI: 10.1101/2020.01.17.909838

Abstract

Abstract Progress in neuroscience is hindered by poor reproducibility of mouse behavior. Here we show that in a visual decision making task, reproducibility can be achieved by automating the training protocol and by standardizing experimental hardware, software, and procedures. We trained 101 mice in this task across seven laboratories at six different research institutions in three countries, and obtained 3 million mouse choices. In trained mice, variability in behavior between labs was indistinguishable from variability within labs. Psychometric curves showed no significant differences in visual threshold, bias, or lapse rates across labs. Moreover, mice across laboratories adopted similar strategies when stimulus location had asymmetrical probability that changed over time. We provide detailed instructions and open-source tools to set up and implement our method in other laboratories. These results establish a new standard for reproducibility of rodent behavior and provide accessible tools for the study of decision making in mice.

Item Type: Paper
Subjects: organism description > animal behavior
organism description > animal behavior > decision making
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Churchland lab
CSHL labs > Zador lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 17 January 2020
Date Deposited: 06 May 2021 13:10
Last Modified: 06 May 2021 13:10
URI: https://repository.cshl.edu/id/eprint/39993

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