Dunn, A. R., Hassell, J. A. (1977) A Novel Method to Map Transcripts Evidence for Homology between an Adenovirus Messenger Rna and Discrete Multiple Regions of the Viral Genome. Cell, 12 (1). pp. 23-36.
Abstract
A method was devised which permits mapping of transcripts by a 2-step hybridization procedure (sandwich hybridization). RNA extracted from [African green monkey kidney CV-1 and human cervical carcinoma HeLa] cells infected with an adenovirus-SV-40 hybrid (Ad2+ND1) was hybridized to [Eco RI, Bgl II, Hind II and Hind III] restriction endonuclease fragments of adenovirus type 2 (Ad2) DNA immobilized on nitrocellulose filters.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription organism description > virus > SV40 |
| CSHL Authors: | |
| Communities: | Cold Spring Harbor Laboratory of Quantitative Biology |
| Depositing User: | Elizabeth Pessala |
| Date: | 1977 |
| Date Deposited: | 07 Apr 2020 15:23 |
| Last Modified: | 07 Apr 2020 15:23 |
| URI: | https://repository.cshl.edu/id/eprint/39004 |
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