Infection of mammalian cells with retroviral shRNAs

Chang, K., Marran, K., Valentine, A., Hannon, G. J. (2013) Infection of mammalian cells with retroviral shRNAs. Cold Spring Harbor Protocols, 2013 (8). pp. 732-733. ISSN 19403402

DOI: 10.1101/pdb.prot076455


Viral infection is a quite simple approach for stably introducing transgenes (e.g., those encoding shorthairpin RNAs [shRNAs]) into the genome. The critical aspects are that the virus and the target cell should be appropriately matched. For example, a virus bearing an ecotropic envelope protein will not infect a human cell line unless the appropriate receptor has been purposefully expressed. VSV-G (vesicular stomatitus virus glycoprotein) pseudotyped viruses have the greatest host range. Nondividing cells can only be infected with lentiviruses, but the additional safety precautions necessary for the use of these tools should dissuade their application to routinely cultured cell lines.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > non-coding RNA
organism description > virus > retrovirus
CSHL Authors:
Communities: CSHL labs > Chang lab
CSHL labs > Hannon lab
Depositing User: Adrian Gomez
Date: 2013
Date Deposited: 22 Nov 2019 20:29
Last Modified: 22 Nov 2019 20:29
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