An Aminoacyl tRNA Synthetase, OKI1, Is Required for Proper Shoot Meristem Size in Arabidopsis

Kitagawa, M., Balkunde, R., Bui, H., Jackson, D. (August 2019) An Aminoacyl tRNA Synthetase, OKI1, Is Required for Proper Shoot Meristem Size in Arabidopsis. Plant Cell Physiol, 60 (11). pp. 2597-2608. ISSN 0032-0781

URL: https://www.ncbi.nlm.nih.gov/pubmed/31393575

DOI: 10.1093/pcp/pcz153

Abstract

In plants, the stem cells that form the shoot system reside within the shoot apical meristem (SAM), which is regulated by feedback signaling between the WUSCHEL (WUS) homeobox protein and CLAVATA (CLV) peptides and receptors. WUS-CLV feedback signaling can be modulated by various endogenous or exogenous factors such as chromatin state, hormone signaling, reactive oxygen species (ROS) signaling and nutrition, leading to a dynamic control of SAM size corresponding to meristem activity. Despite these insights, however, the knowledge of genes that control SAM size is still limited, and in particular the regulation by ROS signaling is only beginning to be comprehended. Here, we report a new gene that functions in SAM size maintenance, OKINA KUKI (OKI1), which is expressed in the SAM and encodes a mitochondrial aspartyl tRNA synthetase (AspRS). oki1 mutants display enlarged SAMs with abnormal expression of WUS and CLV3, and overaccumulation of ROS in the meristem. Our findings support the importance of normal AspRS function in the maintenance of the WUS-CLV3 feedback loop and SAM size.

Item Type:	Paper
Subjects:	organism description > plant > Arabidopsis bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organs, tissues, organelles, cell types and functions > tissues types and functions > meristem organs, tissues, organelles, cell types and functions > organelles, types and functions > mitochondria organs, tissues, organelles, cell types and functions organs, tissues, organelles, cell types and functions > tissues types and functions > signal transduction bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > tRNA organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:	Jackson, David P. Kitagawa, Munenori
Communities:	CSHL labs > Jackson lab
Depositing User:	Matthew Dunn
Date:	8 August 2019
Date Deposited:	12 Aug 2019 18:08
Last Modified:	02 Feb 2024 16:15
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/38185

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