A staging system for the regeneration of a polyp from the aboral physa of the anthozoan Cnidarian Nematostella vectensis

Dunn, M.P., Bossert, P. E., Thomsen, G. H. (November 2013) A staging system for the regeneration of a polyp from the aboral physa of the anthozoan Cnidarian Nematostella vectensis. Developmental Dynamics, 242 (11). pp. 1320-31. ISSN 10588388 (ISSN)

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URL: https://www.ncbi.nlm.nih.gov/pubmed/23913838

DOI: 10.1002/dvdy.24074

Abstract

BACKGROUND: As the sea anemone Nematostella vectensis emerges as a model for studying regeneration, new tools will be needed to assess its regenerative processes and describe perturbations resulting from experimental investigation. Chief among these is the need for a universal set of staging criteria to establish morphological landmarks that will provide a common format for discussion among investigators. RESULTS: We have established morphological criteria to describe stages for rapidly assessing regeneration of the aboral end (physa) of Nematostella. Using this staging system, we observed rates of regeneration that are temperature independent during wound healing and temperature dependent afterward. Treatment with 25 muM lipoic acid delays the progression through wound healing without significantly affecting the subsequent rate of regeneration. Also, while an 11-day starvation before amputation causes only a minimal delay in regeneration, this delay is exacerbated by lipoic acid treatment. CONCLUSIONS: A system for staging the progression of regeneration in amputated Nematostella physa based on easily discernible morphological features provides a standard for the field. This system has allowed us to identify both temperature-dependent and -independent phases of regeneration, as well as a nutritional requirement for normal regenerative progression that is exacerbated by lipoic acid.

Item Type:	Paper
Subjects:	organism description > animal organism description > animal > developmental stage manuals and technical reports organism description > model organism
CSHL Authors:	Dunn, Matthew
Depositing User:	Matthew Dunn
Date:	November 2013
Date Deposited:	09 Jan 2019 21:33
Last Modified:	19 Jul 2021 13:38
PMCID:	PMC6889817
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/37655

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