Keller, W. (1975) Determination of Number of Superhelical Turns in Simian Virus 40 DNA by Gel-Electrophoresis. Proceedings of the National Academy of Sciences of the United States of America, 72 (12). pp. 4876-4880.
Abstract
Closed-circular, superhelical DNA from simian virus 40 (SV40 DNA I) was treated with an excess of DNA-relaxing enzyme in the presence of increasing amounts of ethidium bromide (EtdBr). After removal of the ethidium, each sample consisted of a group of close-circular DNA molecules differing in their number of superhelical turns (tau) around a mean value of tau in a Gaussian-like distribution.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment > assays > gel electrophoresis |
| Communities: | Cold Spring Harbor Laboratory of Quantitative Biology |
| Depositing User: | Elizabeth Pessala |
| Date: | 1975 |
| Date Deposited: | 25 Jan 2019 16:33 |
| Last Modified: | 25 Jan 2019 16:33 |
| PMCID: | PMC388835 |
| URI: | https://repository.cshl.edu/id/eprint/37612 |
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