Sambrook, J., Sugden, B., Keller, W., Sharp, P. A. (December 1973) Transcription of simian virus 40. 3. Mapping of "early" and "late" species of RNA. Proc Natl Acad Sci U S A, 70 (12). pp. 3711-5.
Abstract
To determine the orientation of transcription of the E and L strands of DNA from simian virus 40 (SV40), we used linear DNA prepared by cleavage of superhelical viral DNA by endonuclease R.R(1) from Escherichia coli as a primer.template for DNA polymerase. The resulting molecules, which were labeled only at the 3' end of each DNA strand, were then cleaved with Hemophilus parainfluenzae endonuclease Hpa I
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification organism description > virus |
| CSHL Authors: | |
| Communities: | Cold Spring Harbor Laboratory of Quantitative Biology |
| Depositing User: | Elizabeth Pessala |
| Date: | December 1973 |
| Date Deposited: | 11 Dec 2018 20:06 |
| Last Modified: | 11 Dec 2018 20:06 |
| PMCID: | PMC427312 |
| URI: | https://repository.cshl.edu/id/eprint/37402 |
Actions (login required)
 |
Administrator's edit/view item |