The logic of single-cell projections from visual cortex

Han, Y., Kebschull, J. M., Campbell, R. A. A., Cowan, D., Imhof, F., Zador, A. M., Mrsic-Flogel, T. D. (April 2018) The logic of single-cell projections from visual cortex. Nature, 556 (7699). pp. 51-56. ISSN 0028-0836

Abstract

Neocortical areas communicate through extensive axonal projections, but the logic of information transfer remains poorly understood, because the projections of individual neurons have not been systematically characterized. It is not known whether individual neurons send projections only to single cortical areas or distribute signals across multiple targets. Here we determine the projection patterns of 591 individual neurons in the mouse primary visual cortex using whole-brain fluorescence-based axonal tracing and high-throughput DNA sequencing of genetically barcoded neurons (MAPseq). Projections were highly diverse and divergent, collectively targeting at least 18 cortical and subcortical areas. Most neurons targeted multiple cortical areas, often in non-random combinations, suggesting that sub-classes of intracortical projection neurons exist. Our results indicate that the dominant mode of intracortical information transfer is not based on 'one neuron-one target area' mapping. Instead, signals carried by individual cortical neurons are shared across subsets of target areas, and thus concurrently contribute to multiple functional pathways.

Item Type:	Paper
Subjects:	organism description > animal organs, tissues, organelles, cell types and functions > tissues types and functions > axon organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions organism description > animal > mammal organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal projections organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal projections organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal projections organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent organs, tissues, organelles, cell types and functions > tissues types and functions organs, tissues, organelles, cell types and functions > tissues types and functions > visual cortex
CSHL Authors:	Kebschull, Justus Zador, Anthony M.
Communities:	CSHL labs > Zador lab School of Biological Sciences > Publications
Depositing User:	Matt Covey
Date:	5 April 2018
Date Deposited:	05 Apr 2018 16:07
Last Modified:	20 Feb 2024 20:18
PMCID:	PMC6585423
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/36338

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