Diermeier, S. D., Spector, D. L. (August 2017) Antisense Oligonucleotide-mediated Knockdown in Mammary Tumor Organoids. Bio Protoc, 7 (16). ISSN 2331-8325 (Print)2331-8325
Abstract
Primary mammary tumor organoids grown in 3D are an excellent system to study tumor biology. They resemble the organization and physiology of native epithelia more closely than cancer cell lines grown in 2D, and additionally model interactions with the ECM (Boj et al., 2015; Clevers, 2016; Shamir and Ewald, 2014). Mammary tumor organoids are therefore a promising model system to identify and characterize novel drivers of breast cancer that would be unlikely to be identified using 2D cell lines. Antisense oligonucleotides can be used to efficiently and specifically knockdown target genes in the cell (Bennett et al., 2017). They can be taken up freely by organoids without the need for a transfection agent, making them a convenient tool for routine lab studies and screens.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | 3D cell culture Antisense knockdown Antisense oligonucleotides Breast cancer Mammary tumor Organoids |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > antisense diseases & disorders > cancer > cancer types > breast cancer Investigative techniques and equipment > cell culture bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > oligonucleotide |
| CSHL Authors: | |
| Communities: | CSHL Cancer Center Program > Gene Regulation and Cell Proliferation CSHL labs > Spector lab CSHL Cancer Center Program > Gene Regulation and Inheritance Program |
| Depositing User: | Matt Covey |
| Date: | 20 August 2017 |
| Date Deposited: | 25 Sep 2017 15:37 |
| Last Modified: | 26 Oct 2020 20:16 |
| PMCID: | PMC5602589 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35464 |
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