Nishimura, R. N., Dwyer, B. E., Welch, W., Cole, R., Devellis, J., Liotta, K. (May 1988) The Induction of the Major Heat-Stress Protein in Purified Rat Glial-Cells. Journal of Neuroscience Research, 20 (1). pp. 12-18. ISSN 0360-4012
Abstract
Cultured purified oligodendroglia and astroglia exposed to heat stress (45 °C, 10 or 20 min) synthesized a 68-kDa heat-stress protein, which migrates on twodimensional gel electrophoresis and reacts with a specific monoclonal antibody suggesting it is similar to a major 72-kDa heat-shock protein previously reported in other cell types. This protein was not detected in control glial cultures. Actinomycin D prevented synthesis of this protein demonstrating an absolute requirement for newly synthesized mRNA. The response was prolonged by increasing the period of heat stress from 10 to 20 min. In addition to the 68-kDa HSP protein, the incorporation of radioactivity into 70-, 89-, and 97-kDa proteins was also increased after heating, but in contrast to the 68 kDa protein these proteins appeared to be made in control glial cultures.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification organs, tissues, organelles, cell types and functions > cell types and functions > cell types > glia cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > glia cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > glia cells organism description > animal > mammal > rodent > rat organism description > animal > mammal > rodent > rat |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | May 1988 |
| Date Deposited: | 25 Sep 2017 19:06 |
| Last Modified: | 25 Sep 2017 19:06 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35191 |
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