Four mating-type genes control sexual differentiation in the fission yeast

Kelly, M., Burke, J., Smith, M., Klar, A., Beach, D. (May 1988) Four mating-type genes control sexual differentiation in the fission yeast. Embo J, 7 (5). pp. 1537-47. ISSN 0261-4189

DOI: 10.1002/j.1460-2075.1988.tb02973.x


The mating-type region of fission yeast consists of three components, mat1, mat2-P and mat3-M, each separated by 15 kb. Cell-type is determined by the alternate allele present at mat1, either P in an h+ or M in an h- cell. mat2-P and mat3-M serve as donors of information that is transposed to mat1 during a switch of mating type. We have determined the nucleotide sequence of each component of mat. The P and M specific regions are 1104 and 1128 bp, respectively, and bounded by sequences common to each mating-type cassette (H1; 59 bp and H2; 135 bp). A third sequence is present at mat2-P and mat3-M but absent at mat1 (H3; 57 bp), and may be involved in transcriptional repression of these cassettes. mat1-P and mat1-M each encode two genes (Pc; 118 amino acids, Pi; 159 amino acids, Mc; 181 amino acids and Mi; 42 amino acids). Introduction of opal or frame-shift mutations into the open-reading-frame of each gene revealed that Pc and Mc are necessary and sufficient for mating and confer an h+ or h- mating type respectively. All four genes are required for meiotic competence in an h+/h- diploid. The transcription of each mat gene is strongly influenced by nutritional conditions and full induction was observed only in nitrogen-free medium. The predicted product of the Pi gene contains a region of homology with the homeobox sequence, suggesting that this gene encodes a DNA binding protein that directly regulates the expression of other genes.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence DNA, Fungal/genetics Gene Expression Regulation *Genes, Fungal Genes, Homeobox Meiosis Molecular Sequence Data Peptides/*genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Saccharomycetales/*genetics Schizosaccharomyces/*genetics/growth & development Sequence Homology, Nucleic Acid Transcription, Genetic
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > differentiation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > yeast
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: May 1988
Date Deposited: 12 Oct 2017 17:45
Last Modified: 12 Oct 2017 17:45
PMCID: PMC458406
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