Kelly, M., Burke, J., Smith, M., Klar, A., Beach, D. (May 1988) Four mating-type genes control sexual differentiation in the fission yeast. Embo J, 7 (5). pp. 1537-47. ISSN 0261-4189
Abstract
The mating-type region of fission yeast consists of three components, mat1, mat2-P and mat3-M, each separated by 15 kb. Cell-type is determined by the alternate allele present at mat1, either P in an h+ or M in an h- cell. mat2-P and mat3-M serve as donors of information that is transposed to mat1 during a switch of mating type. We have determined the nucleotide sequence of each component of mat. The P and M specific regions are 1104 and 1128 bp, respectively, and bounded by sequences common to each mating-type cassette (H1; 59 bp and H2; 135 bp). A third sequence is present at mat2-P and mat3-M but absent at mat1 (H3; 57 bp), and may be involved in transcriptional repression of these cassettes. mat1-P and mat1-M each encode two genes (Pc; 118 amino acids, Pi; 159 amino acids, Mc; 181 amino acids and Mi; 42 amino acids). Introduction of opal or frame-shift mutations into the open-reading-frame of each gene revealed that Pc and Mc are necessary and sufficient for mating and confer an h+ or h- mating type respectively. All four genes are required for meiotic competence in an h+/h- diploid. The transcription of each mat gene is strongly influenced by nutritional conditions and full induction was observed only in nitrogen-free medium. The predicted product of the Pi gene contains a region of homology with the homeobox sequence, suggesting that this gene encodes a DNA binding protein that directly regulates the expression of other genes.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence DNA, Fungal/genetics Gene Expression Regulation *Genes, Fungal Genes, Homeobox Meiosis Molecular Sequence Data Peptides/*genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Saccharomycetales/*genetics Schizosaccharomyces/*genetics/growth & development Sequence Homology, Nucleic Acid Transcription, Genetic |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > differentiation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | May 1988 |
| Date Deposited: | 12 Oct 2017 17:45 |
| Last Modified: | 12 Oct 2017 17:45 |
| PMCID: | PMC458406 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35170 |
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