A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout

Erard, Nicolas, Knott, Simon R. V., Hannon, Gregory J. (2017) A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout. Molecular Cell, 67 (2). 348-354.e3. ISSN 1097-2765

URL: https://www.ncbi.nlm.nih.gov/pubmed/28732207
DOI: 10.1016/j.molcel.2017.06.030


Summary We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These strategies have been combined to design and construct a genome-wide, sequence-verified, arrayed CRISPR library. This resource allows single-target or combinatorial genetic screens to be carried out at scale in a multiplexed or arrayed format. By conducting parallel loss-of-function screens, we compare our approach to existing sgRNA design and expression strategies.

Item Type: Paper
Uncontrolled Keywords: CRISPR sgRNA algorithm combinatorial screen expression Cas9 prediction lentivirus library
Subjects: Investigative techniques and equipment > CRISPR-Cas9
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 2017
Date Deposited: 21 Jul 2017 20:49
Last Modified: 06 Jul 2021 19:15
PMCID: PMC5526787
Related URLs:
URI: https://repository.cshl.edu/id/eprint/35069

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