Erard, Nicolas, Knott, Simon R. V., Hannon, Gregory J. (2017) A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout. Molecular Cell, 67 (2). 348-354.e3. ISSN 1097-2765
Abstract
Summary We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These strategies have been combined to design and construct a genome-wide, sequence-verified, arrayed CRISPR library. This resource allows single-target or combinatorial genetic screens to be carried out at scale in a multiplexed or arrayed format. By conducting parallel loss-of-function screens, we compare our approach to existing sgRNA design and expression strategies.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | CRISPR sgRNA algorithm combinatorial screen expression Cas9 prediction lentivirus library |
| Subjects: | Investigative techniques and equipment > CRISPR-Cas9 |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab |
| Depositing User: | Matt Covey |
| Date: | 2017 |
| Date Deposited: | 21 Jul 2017 20:49 |
| Last Modified: | 06 Jul 2021 19:15 |
| PMCID: | PMC5526787 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35069 |
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