White, E., Cipriani, R. (December 1989) Specific disruption of intermediate filaments and the nuclear lamina by the 19-kDa product of the adenovirus E1B oncogene. Proc Natl Acad Sci U S A, 86 (24). pp. 9886-90. ISSN 0027-8424
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Abstract
The 19-kDa protein encoded within the adenovirus E1B gene is essential for transformation by adenovirus and for proper regulation of viral early gene transcription. In order to investigate the biological function of the 19-kDa E1B protein, vectors were constructed to produce the 19-kDa protein in mammalian cells under the direction of heterologous promoters. Surprisingly, during transient expression, the E1B 19-kDa protein specifically associated with and disrupted the organization of intermediate filaments and the nuclear lamina, without disturbing the organization of other cytoskeletal networks. These results directly demonstrate an effect of a viral transforming protein on the cytoskeleton and suggest a role for intermediate filaments and the nuclear lamina in modulation of viral gene expression and the process of oncogenic transformation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenovirus Early Proteins Adenoviruses, Human/*genetics Animals Cell Line Cell Nucleus/*ultrastructure Cell Transformation, Viral Cytoskeleton/*ultrastructure DNA-Binding Proteins/genetics Genes, Structural, Viral Hela Cells/metabolism/ultrastructure Humans Intermediate Filaments/*ultrastructure Molecular Weight Oncogene Proteins, Viral/*genetics/isolation & purification/metabolism *Oncogenes Plasmids Research Support, U.S. Gov't, P.H.S. Restriction Mapping Transcription, Genetic Transfection |
| Subjects: | organism description > virus > adenovirus bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | December 1989 |
| Date Deposited: | 23 Jun 2017 15:24 |
| Last Modified: | 09 Nov 2017 20:45 |
| PMCID: | PMC298607 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34915 |
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