Riabowol, K., Draetta, G., Brizuela, L., Vandre, D., Beach, D. (May 1989) The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells. Cell, 57 (3). pp. 393-401. ISSN 0092-8674
Abstract
A homolog of the fission yeast cdc2-encoded protein kinase (p34) is a component of M phase promoting factor in Xenopus oocytes. The homologous kinase in human HeLa cells is maximally active during mitosis, suggesting a mitotic role in mammalian somatic cells. This has been directly investigated by microinjection of anti-p34 antibodies into serum-stimulated rat fibroblasts. DNA synthesis was unaffected but cell division was quantitatively blocked in injected cells. Injection of antibodies against p13suc1, a component of the p34 kinase complex, did not block mitosis but caused mitotic abnormalities resulting in cells containing multiple micronuclei in the subsequent interphase. p34 localized in the nucleus during interphase. During mitosis, a fraction tightly associated with centrosomes. p13 was more evenly distributed between the nucleus and cytoplasm. These observations demonstrate that cdc2 is a nuclear and centrosomal protein that is required for mitosis in mammalian cells.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals CDC2 Protein Kinase *Cell Cycle Proteins Cell Line Cells, Cultured DNA/biosynthesis Fibroblasts/*metabolism Fungal Proteins/immunology/metabolism Humans Immunohistochemistry Interphase Mice Mitosis/*drug effects Nuclear Proteins/metabolism/pharmacology/*physiology Phosphoproteins/metabolism/pharmacology/*physiology Phosphotransferases/metabolism/pharmacology/*physiology Rats Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. *Schizosaccharomyces pombe Proteins Swine |
| Subjects: | organism description > animal > mammal organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types |
| CSHL Authors: | |
| Communities: | CSHL labs > Beach lab |
| Depositing User: | Gail Sherman |
| Date: | 5 May 1989 |
| Date Deposited: | 27 Jun 2017 15:47 |
| Last Modified: | 27 Jun 2017 15:47 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34897 |
Actions (login required)
 |
Administrator's edit/view item |