Point Mutational Inactivation of the Retinoblastoma Antioncogene

Horowitz, J. M., Yandell, D. W., Park, S. H., Canning, S., Whyte, P., Buchkovich, K., Harlow, E., Weinberg, R. A., Dryja, T. P. (February 1989) Point Mutational Inactivation of the Retinoblastoma Antioncogene. Science, 243 (4893). pp. 937-940. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/2521957
DOI: 10.1126/science.2521957


The retinoblastoma (Rb) antioncogene encodes a nuclear phosphoprotein, p105-Rb, that forms protein complexes with the adenovirus E1A and SV40 large T oncoproteins. A novel, aberrant Rb protein detected in J82 bladder carcinoma cells was not able to form a complex with E1A and was less stable than p105-Rb. By means of a rapid method for the detection of mutations in Rb mRNA, this defective Rb protein was observed to result from a single point mutation within a splice acceptor sequence in J82 genomic DNA. This mutation eliminates a single exon and 35 amino acids from its encoded protein product.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
diseases & disorders > cancer > cancer types > retinoblastoma
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: 17 February 1989
Date Deposited: 28 Jun 2017 15:27
Last Modified: 28 Jun 2017 15:27
Related URLs:
URI: https://repository.cshl.edu/id/eprint/34862

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