Horowitz, J. M., Yandell, D. W., Park, S. H., Canning, S., Whyte, P., Buchkovich, K., Harlow, E., Weinberg, R. A., Dryja, T. P. (February 1989) Point Mutational Inactivation of the Retinoblastoma Antioncogene. Science, 243 (4893). pp. 937-940. ISSN 0036-8075
Abstract
The retinoblastoma (Rb) antioncogene encodes a nuclear phosphoprotein, p105-Rb, that forms protein complexes with the adenovirus E1A and SV40 large T oncoproteins. A novel, aberrant Rb protein detected in J82 bladder carcinoma cells was not able to form a complex with E1A and was less stable than p105-Rb. By means of a rapid method for the detection of mutations in Rb mRNA, this defective Rb protein was observed to result from a single point mutation within a splice acceptor sequence in J82 genomic DNA. This mutation eliminates a single exon and 35 amino acids from its encoded protein product.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene diseases & disorders > cancer > cancer types > retinoblastoma |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | 17 February 1989 |
| Date Deposited: | 28 Jun 2017 15:27 |
| Last Modified: | 28 Jun 2017 15:27 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34862 |
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