Draetta, G., Luca, F., Westendorf, J., Brizuela, L., Ruderman, J., Beach, D. (March 1989) Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF. Cell, 56 (5). pp. 829-38. ISSN 0092-8674
Abstract
In the clam, Spisula, two previously described proteins known as cyclin A and B display the unusual property of selective proteolytic degradation at the end of each mitosis. We show here that clam oocytes and embryos contain a cdc2 protein kinase. This protein kinase is a component of the M phase promoting factor (MPF) in frog eggs and the M phase-specific histone H1 kinase in starfish. Clam cdc2 is found in association with both cyclin A and B, probably not as a trimolecular association, but as separate cdc2/cyclin A and cdc2/cyclin B complexes. Clam cdc2 and the associated cyclins bind to p13suc1-Sepharose. The p13-bound complex, and also anti-cyclin A or B immunoprecipitates, each display cell cycle-dependent histone H1 kinase activity. We suggest that in addition to the cdc2 protein kinase, the cyclins are further components of the M phase promoting factor and that cyclin proteolysis provides the mechanism of MPF inactivation and thus exit from mitosis.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals CDC2 Protein Kinase Clams/*physiology Cyclins Growth Substances/*physiology Invertebrate Hormones/*physiology Maturation-Promoting Factor Meiosis Mitosis Molecular Weight Oocytes/physiology Phosphoproteins/*physiology Protamine Kinase/metabolism Protein Kinases/*physiology Research Support, U.S. Gov't, P.H.S. |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins organism description > animal bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C |
| CSHL Authors: | |
| Communities: | CSHL labs > Beach lab |
| Depositing User: | Gail Sherman |
| Date: | 10 March 1989 |
| Date Deposited: | 31 Jul 2017 17:59 |
| Last Modified: | 31 Jul 2017 17:59 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34840 |
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