Carroll, D., Marshak, D. R. (May 1989) Serum-stimulated cell growth causes oscillations in casein kinase II activity. J Biol Chem, 264 (13). pp. 7345-8. ISSN 1083-351X
Abstract
We have tested the effects of serum-stimulated growth of quiescent WI38 human lung fibroblasts on cellular casein kinase II (CK-II) activity. Using the casein kinase II synthetic substrate RRREEETEEE we find a transient 6-fold elevation in CK-II activity in cell homogenates within 30 min following serum stimulation. Additional cycles of CK-II activation and inactivation are seen at 12 and 24 h after stimulation. The oscillations in CK-II activity are largely independent of de novo protein synthesis, and, thus, are likely to reflect cycles of post-translational activation and inhibition of the cellular kinase pool. In contrast to the activity profile of CK-II, we find that cyclic AMP-dependent protein kinase is rapidly inhibited upon serum-stimulation of WI38 cells. These results demonstrate that CK-II activity is subject to unique cellular regulation during proliferation and are consistent with the postulate that CK-II plays an important role in cell growth.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Anisomycin/pharmacology Casein Kinases *Cell Cycle Cell Line Culture Media Enzyme Activation Growth Substances/pharmacology Humans In Vitro Periodicity Protein Kinases/*metabolism Research Support, U.S. Gov't, P.H.S. |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | 5 May 1989 |
| Date Deposited: | 28 Jul 2017 17:54 |
| Last Modified: | 28 Jul 2017 17:54 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34836 |
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