Buchkovich, K., Duffy, L. A., Harlow, E. (September 1989) The retinoblastoma protein is phosphorylated during specific phases of the cell cycle. Cell, 58 (6). pp. 1097-105. ISSN 1097-4172
Abstract
p105-RB is the product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to act as an inhibitor of cellular proliferation, yet surprisingly it is present in actively dividing cells. To look for changes in p105-RB that may regulate its activity during the cell cycle, we generated synchronized cell populations and followed their progression through the cell cycle. p105-RB is synthesized throughout the cycle, but is phosphorylated in a phase-specific manner. In the G0 and G1 phases of the cell cycle, an unphosphorylated species of the protein is the only detectable form, whereas in the S and G2/M phases, multiple phosphorylated species of p105-RB are detected.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | *Cell Cycle DNA Replication Eye Neoplasms/*genetics Flow Cytometry Hela Cells/cytology/metabolism Humans Immunoblotting Interphase Mitosis Phosphoproteins/biosynthesis/*metabolism Phosphorylation Research Support, U.S. Gov't, P.H.S. Retinoblastoma/*genetics Retinoblastoma Protein *Suppression, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle diseases & disorders > cancer > cancer types > retinoblastoma |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | 22 September 1989 |
| Date Deposited: | 28 Jul 2017 17:22 |
| Last Modified: | 28 Jul 2017 17:22 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34835 |
Actions (login required)
 |
Administrator's edit/view item |