Early Detection of Cancer in Blood Using Single-Cell Analysis: A Proposal

Krasnitz, Alexander, Kendall, Jude, Alexander, Joan, Levy, Dan, Wigler, Michael (July 2017) Early Detection of Cancer in Blood Using Single-Cell Analysis: A Proposal. Trends in Molecular Medicine, 23 (7). pp. 594-603. ISSN 1471-4914

URL: https://www.ncbi.nlm.nih.gov/pubmed/28587830
DOI: 10.1016/j.molmed.2017.05.005


Here, we explore the potential of single-cell genomic analysis in blood for early detection of cancer; we consider a method that screens the presence of recurrent patterns of copy number (CN) alterations using sparse single-cell sequencing. We argue for feasibility, based on in silico analysis of existing single-cell data and cancer CN profiles. Sampling procedures from existing diploid single cells can render data for a cell with any given profile. Sampling from multiple published tumor profiles can interrogate cancer clonality via an algorithm that tests the multiplicity of close pairwise similarities among single-cell cancer genomes. The majority of common solid cancers would be detectable in this manner. As any early detection method must be verifiable and actionable, we describe how further analysis of suspect cells can aid in determining risk and anatomic origin. Future affordability rests on currently available procedures for tumor cell enrichment and inexpensive methods for single-cell analysis.

Item Type: Paper
Uncontrolled Keywords: cancer circulating tumor cells early detection single-cell analysis
Subjects: diseases & disorders > cancer
CSHL Authors:
Communities: CSHL Cancer Center Program > Cancer Genetics
CSHL labs > Krasnitz lab
CSHL labs > Levy lab
CSHL labs > Wigler lab
CSHL Cancer Center Program > Cancer Genetics and Genomics Program
Depositing User: Matt Covey
Date: July 2017
Date Deposited: 05 Jun 2017 20:51
Last Modified: 05 Nov 2020 19:20
PMCID: PMC5502711
Related URLs:
URI: https://repository.cshl.edu/id/eprint/34806

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