RNA interference is essential for cellular quiescence

Roche, B., Arcangioli, B., Martienssen, R. A. (October 2016) RNA interference is essential for cellular quiescence. Science, 354 (6313). ISSN 1095-9203 (Electronic)0036-8075 (Linking)

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URL: https://www.ncbi.nlm.nih.gov/pubmed/27738016

DOI: 10.1126/science.aah5651

Abstract

Quiescent cells play a predominant role in most organisms. Here, we identify RNA interference (RNAi) as a major requirement for quiescence (G0) in Schizosaccharomyces pombe RNAi mutants lose viability at G0-entry and are unable to maintain long-term quiescence. We obtained dcr1Delta G0 suppressors, which mapped to genes involved in chromosome segregation, RNA polymerase-associated factors, and heterochromatin formation. We propose a model in which RNAi promotes RNA polymerase release in cycling and quiescent cells: (i) RNA pol II release mediates heterochromatin formation at centromeres allowing proper chromosome segregation during mitotic growth and G0-entry, and (ii) RNA pol I release prevents heterochromatin formation at rDNA during quiescence maintenance. Our model may account for the co-dependency of RNAi and H3K9 methylation throughout eukaryotic evolution.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle > G0 Phase
CSHL Authors:	Martienssen, Robert A. Roche, Benjamin
Communities:	CSHL Cancer Center Program > Gene Regulation and Cell Proliferation CSHL Cancer Center Program > Gene Regulation and Inheritance Program CSHL labs > Martienssen lab
Depositing User:	Matt Covey
Date:	13 October 2016
Date Deposited:	27 Oct 2016 16:01
Last Modified:	08 Jul 2021 15:39
PMCID:	PMC5858868
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/33820

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