Ellisen, L. W., Palmer, R. E., Maki, R. G., Truong, V. B., Tamayo, P., Oliner, J. D., Haber, D. A. (May 2001) Cascades of transcriptional induction during human lymphocyte activation. Eur J Cell Biol, 80 (5). pp. 321-8. ISSN 0171-9335 (Print)0171-9335 (Linking)
Abstract
Lymphocyte activation is known to be associated with the induction of genes implicated in cytokine signaling and cellular proliferation. High-density microarrays offer the means to monitor global cellular expression profiles, temporal relationships between classes of transcripts, and alterations associated with human disease or immunosuppression. We sought to determine whether microarray analysis would accurately reflect the normal pattern of gene expression following human T cell activation, and whether the complex expression patterns identified could be analyzed to produce a functional profile of lymphocyte activation. We examined a time course of sequential expression profiles for 6,800 cellular transcripts in human lymphocytes activated with concanavalin A. Expression patterns were grouped using clustering analysis and validated using Northern blotting. Genes known to be induced following T cell activation were accurately identified, and the qualitative patterns of gene expression were well correlated between Northern and microarray analyses. Quantitative differences in gene expression levels were less well correlated between these two techniques. Expression profile analysis revealed the sequential induction of groups of functionally similar genes, whose temporal coregulation underscores known cellular events during T cell activation. This functional "fingerprint" of lymphocyte activation may prove useful for comparisons of lymphocyte responses under experimental conditions and in disease states.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Cluster Analysis Gene Expression/immunology Humans Lymphocyte Activation/*genetics Oligonucleotide Array Sequence Analysis T-Lymphocytes/*physiology Transcriptional Activation/*immunology |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression |
| CSHL Authors: | |
| Communities: | CSHL labs > Maki lab |
| Depositing User: | Matt Covey |
| Date: | May 2001 |
| Date Deposited: | 26 Oct 2016 21:01 |
| Last Modified: | 26 Oct 2016 21:01 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/33642 |
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