Kebschull, Justus M, Garcia da Silva, Pedro, Reid, Ashlan P, Peikon, Ian D, Albeanu, Dinu F, Zador, Anthony M (September 2016) High-Throughput Mapping of Single-Neuron Projections by Sequencing of Barcoded RNA. Neuron, 91 (5). pp. 975-987. ISSN 0896-6273 (Public Dataset)
Abstract
Summary Neurons transmit information to distant brain regions via long-range axonal projections. In the mouse, area-to-area connections have only been systematically mapped using bulk labeling techniques, which obscure the diverse projections of intermingled single neurons. Here we describe MAPseq (Multiplexed Analysis of Projections by Sequencing), a technique that can map the projections of thousands or even millions of single neurons by labeling large sets of neurons with random RNA sequences (“barcodes”). Axons are filled with barcode mRNA, each putative projection area is dissected, and the barcode mRNA is extracted and sequenced. Applying MAPseq to the locus coeruleus (LC), we find that individual LC neurons have preferred cortical targets. By recasting neuroanatomy, which is traditionally viewed as a problem of microscopy, as a problem of sequencing, MAPseq harnesses advances in sequencing technology to permit high-throughput interrogation of brain circuits.
| Item Type: | Paper |
|---|---|
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons Investigative techniques and equipment > Whole Brain Circuit Mapping |
| CSHL Authors: | |
| Communities: | CSHL labs > Albeanu lab CSHL labs > Zador lab School of Biological Sciences > Publications |
| Depositing User: | Matt Covey |
| Date: | 7 September 2016 |
| Date Deposited: | 26 Aug 2016 20:54 |
| Last Modified: | 08 Jul 2021 14:56 |
| PMCID: | PMC6640135 |
| Related URLs: | |
| Dataset ID: | |
| URI: | https://repository.cshl.edu/id/eprint/33404 |
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