Renier, Nicolas, Adams, Eliza L, Kirst, Christoph, Wu, Zhuhao, Azevedo, Ricardo, Kohl, Johannes, Autry, Anita E, Kadiri, Lolahon, Umadevi Venkataraju, Kannan, Zhou, Yu, Wang, Victoria X, Tang, Cheuk Y, Olsen, Olav, Dulac, Catherine, Osten, Pavel, Tessier-Lavigne, Marc (June 2016) Mapping of Brain Activity by Automated Volume Analysis of Immediate Early Genes. Cell, 165 (7). pp. 1789-1802. ISSN 0092-8674
Abstract
Summary Understanding how neural information is processed in physiological and pathological states would benefit from precise detection, localization, and quantification of the activity of all neurons across the entire brain, which has not, to date, been achieved in the mammalian brain. We introduce a pipeline for high-speed acquisition of brain activity at cellular resolution through profiling immediate early gene expression using immunostaining and light-sheet fluorescence imaging, followed by automated mapping and analysis of activity by an open-source software program we term ClearMap. We validate the pipeline first by analysis of brain regions activated in response to haloperidol. Next, we report new cortical regions downstream of whisker-evoked sensory processing during active exploration. Last, we combine activity mapping with axon tracing to uncover new brain regions differentially activated during parenting behavior. This pipeline is widely applicable to different experimental paradigms, including animal species for which transgenic activity reporters are not readily available.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > immediate early genes organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits Investigative techniques and equipment > Whole Brain Circuit Mapping |
| CSHL Authors: | |
| Communities: | CSHL labs > Osten lab |
| Depositing User: | Matt Covey |
| Date: | 16 June 2016 |
| Date Deposited: | 31 May 2016 20:09 |
| Last Modified: | 08 Jul 2016 20:53 |
| PMCID: | PMC4912438 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32817 |
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