Kolman, J. L., Taylor, N., Marshak, D. R., Miller, G.
(November 1993)
Serine-173 of the Epstein-Barr-Virus Zebra Protein Is Required for DNA-Binding and Is a Target for Casein Kinase-Ii Phosphorylation.
Proc Natl Acad Sci U S A, 90 (21).
pp. 10115-10119.
ISSN 0027-8424
Abstract
An Epstein-Barr virus-encoded protein, ZEBRA, mediates the switch from latency to the viral lytic life cycle. ZEBRA's domain structure and DNA binding specificity resemble that of cellular transcriptional activators such as c-Fos/c-Jun. We show that ZEBRA, like c-Jun, is phosphorylated by casein kinase II (CKII). The principal site of phosphorylation is serine-173 (S173), five amino acids upstream of the basic DNA recognition domain. CKII phosphorylation abrogated ZEBRA's capacity to bind its target DNA sequences. S173 is a functional component of ZEBRA's DNA binding domain, since mutation of S173 to alanine (S173A) reduced DNA binding in vitro to 10% of wild-type levels. Transcriptional activation of a native viral promoter in vivo by mutant S173A was also reduced markedly. Reversible phosphorylation of S173 is likely to be an important means of regulating ZEBRA's activity in vivo.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
SERUM RESPONSE FACTOR
BZLF1 PROMOTER
EARLY ANTIGEN
CELL-GROWTH
TRANSACTIVATOR
EXPRESSION
ACTIVATION
SITES
TRANSCRIPTION
REGIONS |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > c-fos
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > c-jun
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: |
|
| Communities: |
CSHL labs |
| Depositing User: |
Matt Covey
|
| Date: |
1 November 1993 |
| Date Deposited: |
07 Apr 2016 18:57 |
| Last Modified: |
09 Nov 2017 19:42 |
| PMCID: |
PMC47724 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/32595 |
Actions (login required)
 |
Administrator's edit/view item |
