Mariani, R., Skowronski, J. (June 1993) CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals. Proc Natl Acad Sci U S A, 90 (12). pp. 5549-53. ISSN 0027-8424 (Print)
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Abstract
PCR was used to clone isolates of the human immunodeficiency virus type 1 (HIV-1) nef gene directly from peripheral blood leukocytes of HIV-1-infected individuals. A transient expression system with human CEM T cells was used to assess the effect of nef on CD4 antigen expression on the cell surface. We show that CD4 down-regulation is a frequent property of primary HIV-1 nef alleles. Mutations in conserved amino acid motifs of Nef disrupted CD4 down-regulation. Our observations strongly suggest that CD4 down-regulation reflects a conserved function of nef, which is selected in vivo in human HIV-1 infection. Methodology described here provides quantitative assays to establish whether alterations in nef correlate with the dynamics of disease progression in human AIDS.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Acquired Immunodeficiency Syndrome/ immunology Alleles Amino Acid Sequence Antigens, CD4/ metabolism Base Sequence DNA, Viral/blood/genetics/isolation & purification Down-Regulation Gene Products, nef/biosynthesis/genetics Genes, nef HIV-1/ genetics/ isolation & purification Humans Leukocytes/physiology Molecular Sequence Data Mutation Oligodeoxyribonucleotides Open Reading Frames Polymerase Chain Reaction Research Support, Non-U.S. Gov't |
| Subjects: | diseases & disorders > viral diseases > HIV bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation |
| CSHL Authors: | |
| Communities: | CSHL labs > Skowronski lab |
| Depositing User: | Matt Covey |
| Date: | 15 June 1993 |
| Date Deposited: | 08 Apr 2016 19:54 |
| Last Modified: | 09 Nov 2017 20:00 |
| PMCID: | PMC46758 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32590 |
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