Alfa, C. E., Ducommun, B., Beach, D., Hyams, J. S. (October 1990) Distinct nuclear and spindle pole body population of cyclin-cdc2 in fission yeast. Nature, 347 (6294). pp. 680-2. ISSN 0028-0836 (Print)0028-0836 (Linking)
Abstract
Cyclins, as subunits of the protein kinase encoded by the cdc2 gene are major controlling elements of the eukaryotic cell cycle. The fission yeast Schizosaccharomyces pombe has a B-type cyclin, which is a nuclear protein encoded by the cdc13 gene. Here we demonstrate the presence of two spatially distinct cdc13 cyclin populations in the nucleus of S. pombe, one of which is associated with the mitotic spindle poles. Both populations colocalize with the product of the cdc2 gene (p34cdc2). Treatment of cells with the antimicrotubule drug thiabendazole prevents cyclin degradation and blocks the tyrosine dephosphorylation and activation of cdc2. These results suggest a key regulatory role of the cdc2-cyclin complex in the initiation of mitotic spindle formation and also that mitotic microtubule function is required for cdc2 activation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | CDC2 Protein Kinase/antagonists & inhibitors/*metabolism Cell Nucleus/*metabolism Cyclins/*metabolism Fluorescent Antibody Technique Fungal Proteins/metabolism Mitosis Phosphotyrosine Schizosaccharomyces/drug effects/metabolism/*ultrastructure Spindle Apparatus/*metabolism Thiabendazole/pharmacology Tyrosine/analogs & derivatives/metabolism |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2 organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > mitotic spindle organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Beach lab |
| Depositing User: | Matt Covey |
| Date: | 18 October 1990 |
| Date Deposited: | 05 Apr 2016 18:51 |
| Last Modified: | 05 Apr 2016 18:51 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32278 |
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