Grant, S. G., Jessee, J., Bloom, F. R., Hanahan, D. (June 1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci U S A, 87 (12). pp. 4645-9. ISSN 0027-8424 (Print)0027-8424 (Linking)
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Abstract
Plasmids comprising transgene insertions in four lines of transgenic mice have been retrieved by plasmid rescue into a set of Escherichia coli strains with mutations in different members of the methylation-dependent restriction system (MDRS). Statistical analysis of plasmid rescue frequencies has revealed that the MDRS loci detect differential modifications of the transgene insertions among mouse lines that show distinctive patterns of transgene expression. Plasmids in mice that express hybrid insulin transgenes during development can be readily cloned into E. coli strains carrying mutations in two of the MDRS loci, mcrA and mcrB. In mice in which transgene expression is inappropriately delayed into adulthood, plasmids can only be cloned into E. coli that carry mutations in all known MDRS activities. Differential cloning frequencies in the presence or absence of the various methylation-dependent restriction genes represent a further way to distinguish regions of mammalian chromosomes. These multiply deficient E. coli strains will also facilitate the molecular cloning of modified chromosomal DNA.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Chromosome Mapping DNA/*genetics DNA Transposable Elements Escherichia coli/*genetics Genotype Methylation Mice Mice, Transgenic *Mutation Plasmids/*genetics Restriction Mapping |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation organism description > bacteria > escherichia coli bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > plasmid bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > transposable elements |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | June 1990 |
| Date Deposited: | 05 Apr 2016 14:53 |
| Last Modified: | 09 Nov 2017 20:42 |
| PMCID: | PMC54173 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32275 |
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