Diabetes induced in male transgenic mice by expression of human H-ras oncoprotein in pancreatic beta cells

Efrat, S., Fleischer, N., Hanahan, D. (April 1990) Diabetes induced in male transgenic mice by expression of human H-ras oncoprotein in pancreatic beta cells. Mol Cell Biol, 10 (4). pp. 1779-83. ISSN 0270-7306 (Print)0270-7306 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2181284
DOI: 10.1128/MCB.10.4.1779


Transgenic mice expressing an insulin-promoted H-ras hybrid gene in pancreatic beta cells developed beta-cell degeneration and diabetes. The disease was manifested in male mice by hyperglycemia, glycosuria, and reduced plasma insulin levels, which appeared around 5 months of age and led to premature death. Histological analyses revealed large holes within the islets of Langerhans and a reduced number of beta cells. The destruction of the islets was not associated with an obvious inflammatory activity. Ultrastructural analysis showed extensive engorgement in the endoplasmic reticulum of the residual beta cells from diabetic males. The females carrying the insulin-promoted ras gene did not manifest any of the physiological abnormalities observed in males and showed only minor histological and ultrastructural changes, even at much greater ages.

Item Type: Paper
Uncontrolled Keywords: Animals Cell Line Diabetes Mellitus, Experimental/*genetics/pathology *Genes, ras Humans Immunohistochemistry Insulin/analysis/genetics Islets of Langerhans/metabolism/*pathology/ultrastructure Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Electron Plasmids Proto-Oncogene Proteins/analysis/*genetics Proto-Oncogene Proteins p21(ras) Reference Values Urinary Bladder Neoplasms
Subjects: diseases & disorders > nutritional and metabolic diseases > diabetes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: April 1990
Date Deposited: 05 Apr 2016 14:35
Last Modified: 05 Apr 2016 14:35
PMCID: PMC362285
Related URLs:
URI: https://repository.cshl.edu/id/eprint/32273

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