Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation

Sanchez, Carlos G, Teixeira, Felipe Karam, Czech, Benjamin, Preall, Jonathan B, Zamparini, Andrea L, Seifert, Jessica R K., Malone, Colin D, Hannon, Gregory J, Lehmann, Ruth (December 2015) Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation. Cell Stem Cell, 18 (2). pp. 276-290. ISSN 1934-5909

URL: http://www.ncbi.nlm.nih.gov/pubmed/26669894

DOI: 10.1016/j.stem.2015.11.004

Abstract

Summary Complex regulatory networks regulate stem cell behavior and contributions to tissue growth, repair, and homeostasis. A full understanding of the networks controlling stem cell self-renewal and differentiation, however, has not yet been realized. To systematically dissect these networks and identify their components, we performed an unbiased, transcriptome-wide in vivo RNAi screen in female Drosophila germline stem cells (GSCs). Based on characterized cellular defects, we classified 646 identified genes into phenotypic and functional groups and unveiled a comprehensive set of networks regulating GSC maintenance, survival, and differentiation. This analysis revealed an unexpected role for ribosomal assembly factors in controlling stem cell cytokinesis. Moreover, our data show that the transition from self-renewal to differentiation relies on enhanced ribosome biogenesis accompanied by increased protein synthesis. Collectively, these results detail the extensive genetic networks that control stem cell homeostasis and highlight the intricate regulation of protein synthesis during differentiation.

Item Type:	Paper
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > differentiation organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell organs, tissues, organelles, cell types and functions > organelles, types and functions > ribosome organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
CSHL Authors:	Czech, Benjamin Preall, Jonathan Hannon, Gregory J.
Communities:	CSHL Cancer Center Program > Cancer Genetics and Genomics Program CSHL Cancer Center Program > Gene Regulation and Inheritance Program CSHL labs > Hannon lab CSHL labs > Preall lab
Depositing User:	Matt Covey
Date:	6 December 2015
Date Deposited:	23 Dec 2015 18:18
Last Modified:	13 Jul 2021 16:09
PMCID:	PMC4744108
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/32199

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