Tansey, W. P., Catanzaro, D. F. (May 1991) Sp1 and thyroid hormone receptor differentially activate expression of human growth hormone and chorionic somatomammotropin genes. J Biol Chem, 266 (15). pp. 9805-13. ISSN 0021-9258 (Print)0021-9258 (Linking)
Abstract
Activation of hGH-1 expression is mediated by the pituitary-specific transcription factor GHF-I/Pit-I which binds the 5'-flanking DNA at two sites: I (-96/-70) and II (-134/-106). Although the factor(s) which direct the placental-specific expression of hCS-1 are not known, hCS-1 sequences are transcriptionally active in pituitary cells. In the present study we examined the effects of sequence differences between hGH-1 and hCS-1 5'-flanking DNAs in determining their basal and thyroid hormone-regulated promoter activities. We showed that Sp1 is a major determinant of both hGH-1 and hCS-1 promoter activities and that in hGH-1, binding and activation by Sp1 are modulated by interference from GHF-I/Pit-1 binding at the adjacent site II sequence. A single base which differed in site II of hCS-1 greatly reduced GHF-1/Pit-1 binding and thus facilitated binding and activation by Sp1. Further differences in promoter activity of hGH-1 and hCS-1 sequences were accounted for by a thyroid hormone-responsive element between -62/-48 in the hCS-1 gene. However, induction by T3 was independent of either Sp1 or GH-1/Pit-1 binding in the site II region. These data demonstrate that a small number of base changes between hGH and hCS promoter sequences subserve a number of mechanisms which may differentially modulate the expression of hGH and hCS genes.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence Chloramphenicol O-Acetyltransferase/genetics DNA Fingerprinting DNA-Binding Proteins/metabolism *Gene Expression Regulation Growth Hormone/*genetics Humans Methylation Molecular Sequence Data Placental Lactogen/*genetics Plasmids Receptors, Thyroid Hormone/*metabolism Sp1 Transcription Factor/*metabolism Thyroid Hormones/pharmacology Transcription Factor Pit-1 Transcription Factors/metabolism Transfection |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > hormones > human growth hormone bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > hormones > thyroid hormone bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Tansey lab |
| Depositing User: | Matt Covey |
| Date: | 25 May 1991 |
| Date Deposited: | 10 Dec 2015 20:25 |
| Last Modified: | 10 Dec 2015 20:25 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32148 |
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