Colasanti, J., Tyers, M., Sundaresan, V. (April 1991) Isolation and characterization of cDNA clones encoding a functional p34cdc2 homologue from Zea mays. Proc Natl Acad Sci U S A, 88 (8). pp. 3377-81. ISSN 0027-8424 (Print)0027-8424 (Linking)
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Abstract
We describe the isolation of cDNA clones encoding a p34cdc2 homologue from a higher plant, Zea mays (maize). A full-length cDNA clone, cdc2ZmA, was isolated, sequenced, and shown to complement a cdc28 mutation in Saccharomyces cerevisiae. Comparison of the deduced amino acid sequence of the maize p34cdc2 protein with other homologues showed that it was 64% identical to human p34cdc2 and 63% identical to Schizosaccharomyces pombe and S. cerevisiae p34cdc2 proteins. Studies of expression of the maize cdc2 gene(s) by Northern blot analysis indicated a correlation between the abundance of cdc2 mRNA and the proliferative state of the tissue. Southern blot analysis, as well as isolation of another cDNA clone, cdc2ZmB, which is 96% identical to cdc2ZmA, indicates that maize has multiple cdc2 genes.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern CDC2 Protein Kinase/*genetics *Cell Cycle Cloning, Molecular DNA/genetics Gene Expression Genes Genetic Complementation Test Humans Molecular Sequence Data Polymerase Chain Reaction RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Schizosaccharomyces/genetics Zea mays/*genetics |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2 organism description > plant |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | 15 April 1991 |
| Date Deposited: | 22 Dec 2015 20:42 |
| Last Modified: | 09 Nov 2017 20:19 |
| PMCID: | PMC51450 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32098 |
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