Skowronski, J. (February 1991) Expression of a human immunodeficiency virus type 1 long terminal repeat/simian virus 40 early region fusion gene in transgenic mice. J Virol, 65 (2). pp. 754-62. ISSN 0022-538X (Print)0022-538X (Linking)
Abstract
Three lines of transgenic mice carrying the human immunodeficiency virus type 1 (HIV-1) long terminal repeat fused to the simian virus 40 early region (HIV-1 Tag) were constructed. Expression of the transgenes was reproducibly observed in the lymphoid tissue and skin of all three transgenic lines studied. Interestingly, cell types other than T cells, i.e., B cells and thymic stromal cells, contributed most of the expression detectable in the lymphoid organs. Each transgenic line also displayed a different but consistent pattern of transgene expression in nonlymphoid organs. These individual patterns probably reflect the effects of particular chromosomal integration sites on transcriptional activity of the HIV-1 promoter.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals B-Lymphocytes/metabolism Base Sequence Cloning, Molecular *Genes, Viral HIV Long Terminal Repeat/*genetics HIV-1/*genetics Mice Mice, Inbred Strains Mice, Transgenic Molecular Sequence Data Oligonucleotide Probes Polymerase Chain Reaction Promoter Regions, Genetic RNA, Messenger/genetics RNA, Viral/genetics/isolation & purification Simian virus 40/*genetics T-Lymphocytes/metabolism Transcription, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing diseases & disorders > viral diseases > HIV bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter |
| CSHL Authors: | |
| Communities: | CSHL labs > Skowronski lab |
| Depositing User: | Matt Covey |
| Date: | February 1991 |
| Date Deposited: | 11 Jan 2016 16:40 |
| Last Modified: | 11 Jan 2016 16:40 |
| PMCID: | PMC239815 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32073 |
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