The three-dimensional structure of a DNA duplex containing looped-out bases

Joshua-Tor, L., Rabinovich, D., Hope, H., Frolow, F., Appella, E., Sussman, J. L. (July 1988) The three-dimensional structure of a DNA duplex containing looped-out bases. Nature, 334 (6177). pp. 82-4. ISSN 0028-0836 (Print)0028-0836 (Linking)

DOI: 10.1038/334082a0


Unpaired bases in DNA have been assigned a possible role in the mechanism of frameshift mutagenesis in sequences with repeated base pairs. They also occur in quasipalindromic DNA sequences, which have been implicated in mutagenesis where there are no repeated base pairs, through the formation of single-stranded hairpin loops. The conformation of unpaired bases in DNA has been the subject of numerous thermodynamic as well as high resolution NMR (nuclear magnetic resonance) studies (reviewed in ref. 4). The NMR studies in solution have shown that the duplex of the tridecamer DNA fragment d(CGCAGAATTCGCG) remains intact, and that the unpaired adenosines are stacked into the duplex. Having crystallized this oligonucleotide and determined its structure, we find its conformation in the crystal is close to that of a B-DNA duplex, with the two additional adenosines looped out from the double helix and causing little disruption of the rest of the structure.

Item Type: Paper
Uncontrolled Keywords: Base Composition Base Sequence *Crystallography *Dna Models, Molecular Molecular Sequence Data Mutation *Nucleic Acid Conformation X-Ray Diffraction
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
Investigative techniques and equipment > x ray crystallography
CSHL Authors:
Communities: CSHL labs > Joshua-Tor lab
Depositing User: Matt Covey
Date: 7 July 1988
Date Deposited: 07 Dec 2015 17:30
Last Modified: 07 Dec 2015 17:30
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