Genetic control of morphometric diversity in the maize shoot apical meristem

Leiboff, S., Li, X., Hu, H. C., Todt, N., Yang, J., Li, X., Yu, X., Muehlbauer, G. J., Timmermans, M. C., Yu, J., Schnable, P. S., Scanlon, M. J. (November 2015) Genetic control of morphometric diversity in the maize shoot apical meristem. Nat Commun, 6. p. 8974. ISSN 2041-1723 (Electronic)2041-1723 (Linking)

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DOI: 10.1038/ncomms9974


The maize shoot apical meristem (SAM) comprises a small pool of stem cells that generate all above-ground organs. Although mutational studies have identified genetic networks regulating SAM function, little is known about SAM morphological variation in natural populations. Here we report the use of high-throughput image processing to capture rich SAM size variation within a diverse maize inbred panel. We demonstrate correlations between seedling SAM size and agronomically important adult traits such as flowering time, stem size and leaf node number. Combining SAM phenotypes with 1.2 million single nucleotide polymorphisms (SNPs) via genome-wide association study reveals unexpected SAM morphology candidate genes. Analyses of candidate genes implicated in hormone transport, cell division and cell size confirm correlations between SAM morphology and trait-associated SNP alleles. Our data illustrate that the microscopic seedling SAM is predictive of adult phenotypes and that SAM morphometric variation is associated with genes not previously predicted to regulate SAM size.

Item Type: Paper
Subjects: organism description > plant > maize
organs, tissues, organelles, cell types and functions > tissues types and functions > apical meristem
organism description > plant
CSHL Authors:
Communities: CSHL labs > Timmermans lab
Depositing User: Matt Covey
Date: 20 November 2015
Date Deposited: 25 Nov 2015 20:29
Last Modified: 10 Sep 2019 18:12
PMCID: PMC4673881
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