CCL2 enhances pluripotency of human induced pluripotent stem cells by activating hypoxia related genes

Hasegawa, Y., Tang, D., Takahashi, N., Hayashizaki, Y., Forrest, A. R., Suzuki, H., FANTOM Consortium (2014) CCL2 enhances pluripotency of human induced pluripotent stem cells by activating hypoxia related genes. Sci Rep, 4. p. 5228. ISSN 2045-2322

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URL: http://www.ncbi.nlm.nih.gov/pubmed/24957798

DOI: 10.1038/srep05228

Abstract

Standard culture of human induced pluripotent stem cells (hiPSCs) requires basic Fibroblast Growth Factor (bFGF) to maintain the pluripotent state, whereas hiPSC more closely resemble epiblast stem cells than true naive state ES which requires LIF to maintain pluripotency. Here we show that chemokine (C-C motif) ligand 2 (CCL2) enhances the expression of pluripotent marker genes through the phosphorylation of the signal transducer and activator of transcription 3 (STAT3) protein. Moreover, comparison of transcriptomes between hiPSCs cultured with CCL2 versus with bFGF, we found that CCL2 activates hypoxia related genes, suggesting that CCL2 enhanced pluripotency by inducing a hypoxic-like response.Further, we show that hiPSCs cultured with CCL2 can differentiate at a higher efficiency than culturing withjust bFGF and we show CCL2 can be used in feeder-free conditions [corrected]. Taken together, our finding indicates the novel functions of CCL2 in enhancing its pluripotency in hiPSCs.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression organs, tissues, organelles, cell types and functions > organelles, types and functions > hypoxia organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > pluripotency organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
CSHL Authors:	Gingeras, Thomas R. Davis, Carrie A.
Communities:	CSHL Cancer Center Program > Gene Regulation and Cell Proliferation CSHL labs > Gingeras lab
Depositing User:	Matt Covey
Date:	2014
Date Deposited:	13 Oct 2015 14:27
Last Modified:	09 Nov 2017 21:36
PMCID:	PMC4067614
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/31917

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