Kraus, V. B., Moran, E., Nevins, J. R. (October 1992) Promoter-Specific Transactivation by the Adenovirus-E1a12s Product Involves Separate E1a Domains. Molecular and Cellular Biology, 12 (10). pp. 4391-4399. ISSN 0270-7306
Abstract
Recent studies have shown that the adenovirus E1A12S product can trans-activate transcription by activating the transcription factor E2F. However, E2F cannot be the only target for the E1A12S product, since several cellular promoters have been found to be activated by the E1A12S protein even though they lack E2F sites. Indeed, we now show that activation of the hsp70 promoter by the E1A12S product requires the TATAA sequence. Moreover, activation of the hsp70 promoter requires the N-terminal domain of the EIA protein and does not require the conserved region 2 sequences which are required for the E2F-dependent activation of transcription. We conclude that the targeting of distinct transcription factors, leading to trans-activation of transcription of multiple promoters, involves distinct domains of the EIA proteins that are also required for oncogenic activity.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | E2F TRANSCRIPTION FACTOR RETINOBLASTOMA GENE-PRODUCT CELL NUCLEAR ANTIGEN DNA-BINDING ACTIVITY HUMAN CYCLIN-A FUNCTIONAL DOMAINS TERMINAL REGION MESSENGER-RNAS HSP70 PROMOTER TATA ELEMENTS |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription organism description > virus > adenovirus bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | October 1992 |
| Date Deposited: | 25 Sep 2015 18:51 |
| Last Modified: | 25 Sep 2015 18:51 |
| PMCID: | PMC360363 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31828 |
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