DNA-binding properties of the E1A-associated 300-kilodalton protein

Rikitake, Y., Moran, E. (June 1992) DNA-binding properties of the E1A-associated 300-kilodalton protein. Mol Cell Biol, 12 (6). pp. 2826-36. ISSN 0270-7306 (Print)0270-7306 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1534143
DOI: 10.1128/MCB.12.6.2826


One of the major E1A-associated cellular proteins is a 300-kDa product (p300) that binds to the N-terminal region of the E1A products. The p300 binding site is distinct from sequences involved in binding the retinoblastoma product and other E1A-associated cellular products such as p60-cyclin A and p107. p300 binding to E1A is linked genetically to the enhancer repression function of E1A and the other E1A-mediated gene-regulating functions as well as to the transforming functions of E1A. However, the biochemical properties of p300 have not yet been characterized. We report here that p300 has an intrinsic DNA-binding activity and shows a preferential affinity for specific DNA sequences. The sequences selectively bound by p300 are related to those of a series of enhancer elements that are recognized by NF-kappa B. The direct physical interaction of p300 with enhancer elements provides a biochemical basis for the genetic evidence linking the E1A-mediated enhancer repression function with the p300-binding activity of E1A.

Item Type: Paper
Uncontrolled Keywords: Adenovirus Early Proteins Base Sequence Cross-Linking Reagents DNA-Binding Proteins/isolation & purification/*metabolism *Enhancer Elements, Genetic HeLa Cells Humans Macromolecular Substances Molecular Sequence Data Molecular Weight Oligodeoxyribonucleotides/chemistry Oncogene Proteins, Viral/isolation & purification/*metabolism Protein Binding Transcription Factors/*metabolism Ultraviolet Rays
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: June 1992
Date Deposited: 23 Sep 2015 20:30
Last Modified: 23 Sep 2015 20:30
PMCID: PMC364477
Related URLs:
URI: https://repository.cshl.edu/id/eprint/31824

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