Human p53 inhibits growth in Schizosaccharomyces pombe

Bischoff, J. R., Casso, D., Beach, D. (April 1992) Human p53 inhibits growth in Schizosaccharomyces pombe. Mol Cell Biol, 12 (4). pp. 1405-11. ISSN 0270-7306 (Print)0270-7306 (Linking)

DOI: 10.1128/MCB.12.4.1405


Overexpression of wild-type p53 in mammalian cells blocks growth. We show here that the overexpression of wild-type human p53 in the fission yeast Schizosaccharomyces pombe also blocks growth, whereas the overexpression of mutant forms of p53 does not. The p53 polypeptide is located in the nucleus and is phosphorylated at both the cdc2 site and the casein kinase II site in S. pombe. A new dominant mutation of p53, resulting in the change of a cysteine to an arginine at amino acid residue 141, was identified. The results presented here demonstrate that S. pombe could provide a simple system for studying the mechanism of action of human p53.

Item Type: Paper
Uncontrolled Keywords: Alleles Base Sequence Cell Division/genetics *Gene Expression Regulation Genes, Dominant/genetics Genome, Human Humans Molecular Sequence Data Mutation Phosphorylation Schizosaccharomyces/genetics/*growth & development Tumor Suppressor Protein p53/*genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p53
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Matt Covey
Date: April 1992
Date Deposited: 23 Sep 2015 19:26
Last Modified: 23 Sep 2015 19:26
PMCID: PMC369581
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