Rothstein, J. L., Johnson, D., DeLoia, J. A., Skowronski, J., Solter, D., Knowles, B. (July 1992) Gene expression during preimplantation mouse development. Genes Dev, 6 (7). pp. 1190-201. ISSN 0890-9369 (Print)0890-9369 (Linking)
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Abstract
To develop a resource for the identification and isolation of genes expressed in the early mammalian embryo, large and representative cDNA libraries were constructed from unfertilized eggs, and two-cell, eight-cell, and blastocyst-stage mouse embryos. Using these libraries, we now report the first stages at which the cytokines interleukin (IL)-6, IL-1 beta, and interferon (IFN)-gamma are transcribed in the developing embryo and the presence of IL-7 transcripts in the unfertilized egg. Transcripts for IL-1 alpha, -2, -3, -4, or -5 were not detected at these stages. To identify novel genes expressed on activation of the embryonic genome, the egg and eight-cell stage-specific cDNA libraries were subtracted from the two-cell library, yielding a specialized cDNA library enriched for transcripts expressed at the two-cell stage. Sequence and Southern blot analysis of several of these cDNAs expressed predominantly at the two-cell stage of embryogenesis revealed them to be from novel genes, thereby providing the first molecular tools with which to approach the study of gene expression in the early mammalian embryo.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Base Sequence Blotting, Southern Cloning, Molecular Cytokines/*genetics Dna Embryonic Development/*genetics Embryonic and Fetal Development/*genetics Female *Gene Expression Regulation Gene Library Mice Molecular Sequence Data Polymerase Chain Reaction Pregnancy Transcription, Genetic |
| Subjects: | organism description > animal > developmental stage organism description > animal > developmental stage > embryo organs, tissues, organelles, cell types and functions > tissues types and functions > embryogenesis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression organism description > animal > mammal > rodent > mouse |
| CSHL Authors: | |
| Communities: | CSHL labs > Skowronski lab |
| Depositing User: | Matt Covey |
| Date: | July 1992 |
| Date Deposited: | 29 Sep 2015 16:30 |
| Last Modified: | 03 Nov 2017 20:49 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31797 |
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