Bessho, Y., Nawa, H., Nakanishi, S. (January 1994) Selective up-Regulation of an Nmda Receptor Subunit Messenger-Rna in Cultured Cerebellar Granule Cells by K+-Induced Depolarization and Nmda Treatment. Neuron, 12 (1). pp. 87-95. ISSN 0896-6273
Abstract
High KCl or NMDA treatment promotes the survival of cultured neonatal cerebellar granule cells, and these cells become sensitive to NMDA toxicity after prolonged K+ depolarization. Following both treatments, the NMDA receptor increases, as assessed by fura-2 fluorescence analysis of NMDA receptor-mediated intracellular Ca2+ increase. Northern analysis indicates that both treatments specifically up-regulate NMDAR2A subunit mRNA through an increase in resting intracellular Ca2+ concentration. Antisense oligonucleotide analysis further indicates that NMDAR2A mRNA up-regulation is responsible for NMDA receptor induction. Our results demonstrate that regulation of a specific NMDA receptor subunit mRNA governs NMDA receptor induction, which is thought to play an important role in granule cell survival and death.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | GLUTAMATE RECEPTORS MOLECULAR DIVERSITY EXPRESSION DISTINCTION SURVIVAL SUBTYPES NEURONS BRAIN |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | January 1994 |
| Date Deposited: | 04 May 2015 15:57 |
| Last Modified: | 04 May 2015 15:57 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31480 |
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